Product Name: VEGFR3-2
Product Number: AB-NK064-3
Size: 25 µg      Price:89.00
      $US
Target Full Name: Vascular endothelial growth factor receptor 3 ; Vascular endothelial growth factor receptor-protein-tyrosine kinase 3

Target Alias: Fms-related tyrosine kinase 4; PCL; Tyrosine-protein kinase receptor FLT4; FLT41; VEGFR3; VGFR3; VGR3; CCDS4457.1; ENSG00000037280

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK064-3

Antibody Target Type: Pan-specific

Protein UniProt: P35916

Protein SigNET: P35916

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human VEGFR3 (Flt4) sequence peptide Cat. No.: PE-01AVQ80

Antibody Immunogen Sequence: CIQRFRESTEVIVHEN

Antibody Immunogen Description: Corresponds to amino acid residues I316 to N330

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.5 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Strong immunoreactivity with recombinant human VEGFR3 on protein dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 160-175 kDa.

Antibody Specificity: Medium
Antibody Cross Reactivity: Weak immunoreactivity on protein dot blots with recombinant human VEGFR2, and no immunoreactivity with VEGFR1.

Related Product 1: VEGFR3-2 blocking peptide

Related Product 2: VEGFR3 pan-specific antibody (Cat. No.: AB-NK064-2P)

Related Product 3: VEGFR3-1 pan-specific antibody (Cat. No.: AB-NK064-2)

Related Product 4: VEGFR3-pY1068 phosphosite-specific antibody (Cat. No.: AB-PK853)

Scientific Background: VEGFR3 (Flt4) is a protein-tyrosine kinase of the TK group and VEGFR family. It is a receptor for vascular endothelial growth factors (VEGF) C and D that is essential in angiogenesis and vasculogenesis. It is activated by binding VEGF-C and VEGF-D, which induce dimerization and autophosphorylation. VEGFR3 plays critical roles in some lymphangiogenetic mechanisms in cancer and metastasis. It is expressed in acute myeloid leukemia and other types of cancer, and are related to tumour angiogenesis and efficacy of traditional therapies.
Figure 1. Dot blotting VGFR3-2 antibody with recombinant purified proteins.