Product Name: VEGFR3-pY1068
Product Number: AB-PK853
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Vascular endothelial growth factor receptor 3 ; Vascular endothelial growth factor receptor-protein-tyrosine kinase 3
Target Alias: Fms-related tyrosine kinase 4; PCL; Tyrosine-protein kinase receptor FLT4; FLT41; VEGFR3; VGFR3; VGR3; CCDS4457.1; ENSG00000037280
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK853
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y1068
Protein UniProt: P35916
Protein SigNET: P35916
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Target Alias: Fms-related tyrosine kinase 4; PCL; Tyrosine-protein kinase receptor FLT4; FLT41; VEGFR3; VGFR3; VGR3; CCDS4457.1; ENSG00000037280
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK853
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y1068
Protein UniProt: P35916
Protein SigNET: P35916
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human VEGFR3 (Flt4) sequence peptide Cat. No.: PE-04AMK99
Antibody Immunogen Sequence: DPD(pY)VRK(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D1065 to K1071; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Immunogen Sequence: DPD(pY)VRK(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D1065 to K1071; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots. Strong immunoreactivity with recombinant human VEGFR3 on protein dot blots.
Antibody Species Reactivity: Human
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots. Strong immunoreactivity with recombinant human VEGFR3 on protein dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 160-175 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: Weak immunoreactivity on protein dot blots with recombinant human VEGFR2 and VEGFR3. Almost no significant cross-reactives in T98G and sea star oocytes, although a strong 80 kDa cross-reactive protein is detected in sea star oocytes.
Related Product 1: VEGFR3-pY1068 blocking peptide
Related Product 2: VEGFR3 pan-specific antibody (Cat. No.: AB-NK064-2P)
Related Product 3: VEGFR3-1 pan-specific antibody (Cat. No.: AB-NK064-2)
Related Product 4: VEGFR3-2 pan-specific antibody (Cat. No.: AB-NK064-3)
Antibody Specificity: High-very high
Antibody Cross Reactivity: Weak immunoreactivity on protein dot blots with recombinant human VEGFR2 and VEGFR3. Almost no significant cross-reactives in T98G and sea star oocytes, although a strong 80 kDa cross-reactive protein is detected in sea star oocytes.
Related Product 1: VEGFR3-pY1068 blocking peptide
Related Product 2: VEGFR3 pan-specific antibody (Cat. No.: AB-NK064-2P)
Related Product 3: VEGFR3-1 pan-specific antibody (Cat. No.: AB-NK064-2)
Related Product 4: VEGFR3-2 pan-specific antibody (Cat. No.: AB-NK064-3)
Scientific Background: VEGFR3 (Flt4) is a protein-tyrosine kinase of the TK group and VEGFR family. It is a receptor for vascular endothelial growth factors (VEGF) C and D that is essential in angiogenesis and vasculogenesis. It is activated by binding VEGF-C and VEGF-D, which induce dimerization and autophosphorylation. VEGFR3 plays critical roles in some lymphangiogenetic mechanisms in cancer and metastasis. It is expressed in acute myeloid leukemia and other types of cancer, and are related to tumour angiogenesis and efficacy of traditional therapies.
Figure 3. Dot blotting VEGFR3-pY1068 antibody with recombinant purified proteins.
Figure 2. Identification of phosphosites related to VEGFR3-pY1068.
© Kinexus Bioinformatics Corporation 2017