Product Name: Ksr1
Product Number: AB-NK090
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Protein-serine kinase suppressor of Ras 1
Target Alias: CAP kinase; Ceramide-activated protein kinase; HB; HB protein; Ksr; Ksr1; Kinase suppressor of ras 1; Kinase suppressor of RAS-1; RSU2; ENSG00000141068
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK090
Antibody Target Type: Pan-specific
Protein UniProt: Q8IVT5
Protein SigNET: Q8IVT5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Ksr1 sequence peptide
Target Alias: CAP kinase; Ceramide-activated protein kinase; HB; HB protein; Ksr; Ksr1; Kinase suppressor of ras 1; Kinase suppressor of RAS-1; RSU2; ENSG00000141068
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK090
Antibody Target Type: Pan-specific
Protein UniProt: Q8IVT5
Protein SigNET: Q8IVT5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Ksr1 sequence peptide
Antibody Immunogen Sequence: EKLPKLNRRLSHPGHFWKSC
Antibody Immunogen Description: Corresponds to amino acid residues E829 to S847; C-terminus
Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues E829 to S847; C-terminus
Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human; Mouse; Rat
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human; Mouse; Rat
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 105-120 kDa.
Related Product 1: Ksr1-pS406 phosphosite-specific antibody (Cat. No.: AB-PK675)
Related Product 2: Ksr2-pS490 phosphosite-specific antibody (Cat. No.: AB-PK676)
Related Product 1: Ksr1-pS406 phosphosite-specific antibody (Cat. No.: AB-PK675)
Related Product 2: Ksr2-pS490 phosphosite-specific antibody (Cat. No.: AB-PK676)
Scientific Background: Ksr1 (kinase suppressor of Ras 1) is a protein-serine/threonine kinase of the TKL group and RAF family. It is a Raf-related pseudokinase and may directly mediate Raf activation. It acts as a scaffold for the Raf/MEK/ERK kinase cascade. No specific mutations have been directly implicated in oncogenesis although loss of Ksr1 function will blunt the Ras pathway and therefore attenuate its oncogenic potential.
© Kinexus Bioinformatics Corporation 2017