Product Name: Ksr2-pS490
Product Number: AB-PK676
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Kinase suppressor of Ras 2
Target Alias: FLJ25965; AC026678_EPK
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK676
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S490
Protein UniProt: Q6VAB6
Protein SigNET: Q6VAB6
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Ksr2 sequence peptide Cat. No.: PE-04ANF95
Target Alias: FLJ25965; AC026678_EPK
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK676
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S490
Protein UniProt: Q6VAB6
Protein SigNET: Q6VAB6
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Ksr2 sequence peptide Cat. No.: PE-04ANF95
Antibody Immunogen Sequence: PRY(pS)DLH(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues P487 to H493; In the region between the Ksr1-SAM and kinase catalytic domains. This is the major in vivo phosphorylation site in Krs2.
Antibody Immunogen Description: Corresponds to amino acid residues P487 to H493; In the region between the Ksr1-SAM and kinase catalytic domains. This is the major in vivo phosphorylation site in Krs2.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 97-102 kDa.
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 97-102 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactivities in MCF7and T98G cells or in sea star oocytes, except for ~50 and ~27 kDa proteins in MCF7 cells.
Related Product 1: Ksr2-pS490 blocking peptide
Related Product 2: Ksr1 pan-specific antibody (Cat. No.: AB-NK090)
Related Product 3: Ksr1-pS406 phosphosite-specific antibody (Cat. No.: AB-PK675)
Antibody Cross Reactivity: No significant cross-reactivities in MCF7and T98G cells or in sea star oocytes, except for ~50 and ~27 kDa proteins in MCF7 cells.
Related Product 1: Ksr2-pS490 blocking peptide
Related Product 2: Ksr1 pan-specific antibody (Cat. No.: AB-NK090)
Related Product 3: Ksr1-pS406 phosphosite-specific antibody (Cat. No.: AB-PK675)
Scientific Background: Ksr2 (kinase suppressor of Ras 2) is a protein-serine/threonine kinase of the TKL group and RAF family. It functions as a scaffold protein that connects MEK and Raf isoforms. The Ksr2 protein has very low kinase catalytic activity, but it can phosphorylate MAP2K1 in vitro with low efficiency at several serine and threonine residues. Ksr2 functions to inhibit MAP3K3-mediated activation of ERK, JNK, and NF-kappa-B signalling pathways, thus blocking MAP3K-mediated production of interleukin-8 (IL-8). In addition, Ksr2 interacts with AMPK (the cellular energy sensor) to maintain energy homeostasis and glucose utilization.
Figure 1. Epitope mapping of Ksr2-pS490 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to Ksr2-pS490.
© Kinexus Bioinformatics Corporation 2017