Product Name: MEK2-3
Product Number: AB-NK100-6
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: MAPK/ERK protein-serine kinase 2 (MKK2); Dual specificity mitogen-activated protein kinase kinase 2
Target Alias: ERK activator kinase 2; MAP kinase kinase 2; MAP2K2; MAPK,ERK kinase 2; MAPKK2; MKK2; MP2K2; PRKMK2EC; MEK2; PRKMK2; CCDS12120.1; ENSG00000126934
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK100-6
Antibody Target Type: Pan-specific
Protein UniProt: P36507
Protein SigNET: P36507
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MEK2 (MKK2, MAP2K2) sequence peptide Cat. No.: PE-01AVX90
Target Alias: ERK activator kinase 2; MAP kinase kinase 2; MAP2K2; MAPK,ERK kinase 2; MAPKK2; MKK2; MP2K2; PRKMK2EC; MEK2; PRKMK2; CCDS12120.1; ENSG00000126934
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK100-6
Antibody Target Type: Pan-specific
Protein UniProt: P36507
Protein SigNET: P36507
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MEK2 (MKK2, MAP2K2) sequence peptide Cat. No.: PE-01AVX90
Antibody Immunogen Sequence: CTLRLNQPGTPTRTAV
Antibody Immunogen Description: Corresponds to amino acid residues T386 to V400; C-terminus
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues T386 to V400; C-terminus
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with recombinant human MEK2 on protein dot blots.
Antibody Species Reactivity: Human; Mouse
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with recombinant human MEK2 on protein dot blots.
Antibody Species Reactivity: Human; Mouse
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 43-50 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human MEK1, MKK4 and MKK6.
Related Product 1: MEK2-3 blocking peptide
Related Product 2: MEK2 pan-specific antibody (Cat. No.: AB-NK100-4P)
Related Product 3: MEK2-1 pan-specific antibody (Cat. No.: AB-NK100-5)
Related Product 4: MEK2-2 pan-specific antibody (Cat. No.: AB-NK100-4)
Related Product 5: MEK2-NT (MEK2-4) pan-specific antibody (Cat. No.: AB-NK100-3)
Antibody Specificity: Very high
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human MEK1, MKK4 and MKK6.
Related Product 1: MEK2-3 blocking peptide
Related Product 2: MEK2 pan-specific antibody (Cat. No.: AB-NK100-4P)
Related Product 3: MEK2-1 pan-specific antibody (Cat. No.: AB-NK100-5)
Related Product 4: MEK2-2 pan-specific antibody (Cat. No.: AB-NK100-4)
Related Product 5: MEK2-NT (MEK2-4) pan-specific antibody (Cat. No.: AB-NK100-3)
Scientific Background: MEK2 (MAP2K2) is a Dual specificity protein kinase of the STE group and STE7 family. Phosphorylation of S222 and S226 increases its phosphotransferase activity. Phosphorylation of S216 inhibits phosphotransferase activity. It controls the concomitant phosphorylation of the threonine and tyrosine in a Thr-Glu-Tyr sequence in MAP kinases. It particularly targets and activates the ERK1 and ERK2 MAP kinases.
Figure 1. Dot blotting MEK2-3 antibody with recombinant purified proteins.
© Kinexus Bioinformatics Corporation 2017