Product Name: Ron-1
Product Number: AB-NK161-2
Size: | 25 µg | | Price: | 89.00 |
| | | $US | |
Target Full Name: Macrophage-stimulating protein receptor alpha chain
Target Alias: CD136; CD136 antigen; CDW136; C-met-related tyrosine kinase; Kinase RON; Macrophage stimulating 1 receptor; Macrophage-stimulating protein receptor precursor; MSP receptor; MST1R; PTK8; STK; CCDS2807.1; ENSG00000164078
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK161-2
Antibody Target Type: Pan-specific
Protein UniProt: Q04912 Protein SigNET: Q04912 Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Ron (RONa) sequence peptide Cat. No.: PE-01AVD95
Antibody Immunogen Sequence: CLRDLDSALLAEVKDV
Antibody Immunogen Description: Corresponds to amino acid residues R1060 to V1073
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium-strong immunoreactivity with recombinant human Ron on protein dot blots.
Antibody Species Reactivity: Human; Mouse
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 150-220 kDa.
Scientific Background: Ron (MST1R) is a protein-tyrosine kinase of the TK group and Met family. It is a receptor kinase that binds macrophage stimulating factor 1 (MST1, MSF) to propagate a cell migration, survival, or differentiation signal. This kinase is highly expressed and widely distributed in most tested human tissues. Phosphorylation of Ron at Y1198 and Y1288 increases phosphotransferase activity. Phosphorylation at S1394 induces interaction with 14-3-3 beta. Ron interacts with the HYAL2 receptor protein, rendering it functionally inactive. HYAL2 is a candidate tumour-suppressor glycosylphosphatidylinositol-anchored cell-surface protein that serves as an entry receptor for jaagsiekte retrovirus, a virus that causes contagious lung cancer in sheep that is morphologically similar to human bronchioloalveolar carcinoma. It was shown that Ron liberated from the association with HYAL2 becomes functionally active and activates the Akt and mitogen-activated protein kinase pathways. Ron has also been linked with the development of breast, head and neck, ovarian, renal and breast cancers, and pulmonary adenomas.
Figure 1. Dot blotting Ron-1 antibody with recombinant purified proteins.