Product Name: JNK3-1
Product Number: AB-NK197-2
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Jun N-terminus protein-serine kinase (stress-activated protein kinase (SAPKb)) 3
Target Alias: c-Jun N-terminal kinase 3; FLJ12099; FLJ33785; JNK3A; JNK3-alpha-2; Kinase JNK3; MAP kinase p49 3F12; MAPK10; MGC50974; MK10; P493F12; p54bSAPK; PRKM10; Stress-activated protein kinase JNK3; PRKM10; CCDS3613.1; ENSG00000109339
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK197-2
Antibody Target Type: Pan-specific
Protein UniProt: P53779
Protein SigNET: P53779
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Target Alias: c-Jun N-terminal kinase 3; FLJ12099; FLJ33785; JNK3A; JNK3-alpha-2; Kinase JNK3; MAP kinase p49 3F12; MAPK10; MGC50974; MK10; P493F12; p54bSAPK; PRKM10; Stress-activated protein kinase JNK3; PRKM10; CCDS3613.1; ENSG00000109339
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK197-2
Antibody Target Type: Pan-specific
Protein UniProt: P53779
Protein SigNET: P53779
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human JNK3 (MAPK10) sequence peptide Cat. No.: PE-01AXQ99
Antibody Immunogen Sequence: CAGLTFPKLFPDSLFP
Antibody Immunogen Description: Corresponds to amino acid residues A305 to P319; Pre-kinase last alpha-chain
Antibody Immunogen Sequence: CAGLTFPKLFPDSLFP
Antibody Immunogen Description: Corresponds to amino acid residues A305 to P319; Pre-kinase last alpha-chain
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 48-54 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human JNK1 and JNK2.
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 48-54 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human JNK1 and JNK2.
Related Product 1: JNK3-1 blocking peptide
Related Product 2: JNK1-1 pan-specific antibody (Cat. No.: AB-NK217-2)
Related Product 3: JNK1-2 pan-specific antibody (Cat. No.: AB-NK217-3)
Related Product 4: JNK1-pY185 phosphosite-specific antibody (Cat. No.: AB-PK670)
Related Product 5: JNK2-1 pan-specific antibody (Cat. No.: AB-NK189-2)
Related Product 6: SAPKa/b (JNK2-4) pan-specific antibody (Cat. No.: AB-NK196)
Related Product 7: JNK3-2 pan-specific antibody (Cat. No.: AB-NK197-3)
Related Product 8: JNK3-3 pan-specific antibody (Cat. No.: AB-NK197-4)
Related Product 9: JNKSelectideA - JNK1 protein kinase substrate peptide
Related Product 2: JNK1-1 pan-specific antibody (Cat. No.: AB-NK217-2)
Related Product 3: JNK1-2 pan-specific antibody (Cat. No.: AB-NK217-3)
Related Product 4: JNK1-pY185 phosphosite-specific antibody (Cat. No.: AB-PK670)
Related Product 5: JNK2-1 pan-specific antibody (Cat. No.: AB-NK189-2)
Related Product 6: SAPKa/b (JNK2-4) pan-specific antibody (Cat. No.: AB-NK196)
Related Product 7: JNK3-2 pan-specific antibody (Cat. No.: AB-NK197-3)
Related Product 8: JNK3-3 pan-specific antibody (Cat. No.: AB-NK197-4)
Related Product 9: JNKSelectideA - JNK1 protein kinase substrate peptide
Scientific Background: JNK3 (MAPK10) is a protein-serine/threonine kinase of the CMGC group and MAPK family. Phosphorylation at T221 and Y223, by MAP2K4 and MAP2K7. MAP2K7, increases phosphotransferase activity. MAP2K7 phosphorylates JNK3 on T221 causing a conformational change and a large increase in Vmax. MAP2K4 then phosphorylates Y223 resulting in a further increase in Vmax. Phosphorylation at T131 inhibits phosphotransferase activity. It is inhibited by dual specificity phosphatases, such as DUSP1 and by HDAC9. The near absence of reported somatic mutations in this protein indicates that it may function as a tumour requiring protein (TRP) in many human tumours.
Figure 1. Dot blotting JNK3-1 antibody with recombinant purified proteins.
© Kinexus Bioinformatics Corporation 2017