Product Name: JNK2-4 (SAPKa/b)
Product Number: AB-NK196
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Jun N-terminus protein-serine kinase (stress-activated protein kinase (SAPK)) 2
Target Alias: c-Jun N-terminal kinase 2; JNK2alpha; JNK2-alpha-2; JNK2A; JNK2B; JNK2beta; JNK-55; Jun kinase; Kinase JNK2; MAPK9; MK09; p54a; PRKM9; Stress-activated protein kinase JNK2; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; CCDS4453.1; ENSG00000050748
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK196
Antibody Target Type: Pan-specific
Protein UniProt: P45984
Protein SigNET: P45984
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Target Alias: c-Jun N-terminal kinase 2; JNK2alpha; JNK2-alpha-2; JNK2A; JNK2B; JNK2beta; JNK-55; Jun kinase; Kinase JNK2; MAPK9; MK09; p54a; PRKM9; Stress-activated protein kinase JNK2; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; CCDS4453.1; ENSG00000050748
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK196
Antibody Target Type: Pan-specific
Protein UniProt: P45984
Protein SigNET: P45984
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human JNK2 (MAPK9) sequence peptide
Antibody Immunogen Sequence: Full length recombinant SAPKa expressed as a GST-fusion protein
Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Sequence: Full length recombinant SAPKa expressed as a GST-fusion protein
Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.25 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with 46 and 54 kDa isoforms of JNK2.
Antibody Concentration: 0.25 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with 46 and 54 kDa isoforms of JNK2.
Antibody Species Reactivity: Human; Mouse; Rat
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 48-54 kDa.
Antibody Cross Reactivity: Medium immunoreactivity with JNK1 and JNK3.
Related Product 1: JNK1-1 pan-specific antibody (Cat. No.: AB-NK217-2)
Related Product 2: JNK1-2 pan-specific antibody (Cat. No.: AB-NK217-3)
Related Product 3: JNK1-pY185 phosphosite-specific antibody (Cat. No.: AB-PK670)
Related Product 4: JNK2-1 pan-specific antibody (Cat. No.: AB-NK189-2)
Related Product 5: JNK2-2 pan-specific antibody (Cat. No.: AB-NK189-3)
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 48-54 kDa.
Antibody Cross Reactivity: Medium immunoreactivity with JNK1 and JNK3.
Related Product 1: JNK1-1 pan-specific antibody (Cat. No.: AB-NK217-2)
Related Product 2: JNK1-2 pan-specific antibody (Cat. No.: AB-NK217-3)
Related Product 3: JNK1-pY185 phosphosite-specific antibody (Cat. No.: AB-PK670)
Related Product 4: JNK2-1 pan-specific antibody (Cat. No.: AB-NK189-2)
Related Product 5: JNK2-2 pan-specific antibody (Cat. No.: AB-NK189-3)
Related Product 6: JNK2-3 pan-specific antibody (Cat. No.: AB-NK189-4)
Related Product 7: JNK3-1 pan-specific antibody (Cat. No.: AB-NK197-2)
Related Product 8: JNK3-3 pan-specific antibody (Cat. No.: AB-NK197-4)
Related Product 9: JNKSelectideA - JNK1 protein kinase substrate peptide
Related Product 10: JNKSubtide - JNK1 (MAPK8) protein kinase substrate peptide
Related Product 7: JNK3-1 pan-specific antibody (Cat. No.: AB-NK197-2)
Related Product 8: JNK3-3 pan-specific antibody (Cat. No.: AB-NK197-4)
Related Product 9: JNKSelectideA - JNK1 protein kinase substrate peptide
Related Product 10: JNKSubtide - JNK1 (MAPK8) protein kinase substrate peptide
Scientific Background: JNK2 (MAPK9) is a protein-serine/threonine kinase of the CMGC group and MAPK family. It is involved in the control of cell differentiation, transformation, migration, proliferation, and programmed cell death in response to stress and cytokines. It has also been implicated in preventing ubiquitination of p53.Phosphorylation of T183 and Y185 by either of two dual specificity kinases, MAP2K4 and MAP2K7, increases phosphotransferase activity. Phosphorylation of S129 also increases phosphotransferase activity. It is nhibited by dual specificity phosphatases, such as DUSP1, which target the T183 and Y185 activatory phosphorylation sites. JNK2 has been shown, in a cancer cell line to have a role in breast cancer cell motility.
Figure 1. Dot blotting JNK2-4 antibody with recombinant purified proteins.
© Kinexus Bioinformatics Corporation 2017