Product Name: SIK2-3
Product Number: AB-NK249-3
Size: 25 µg      Price:89.00
      $US
Target Full Name: Salt-inducible serine/threonine-protein kinase SIK2; Serine-threonine-protein kinase SNF1-like kinase 2

Target Alias: DKFZp434K1115; KIAA0781; LOH11CR1I; Salt-inducible kinase 2; Salt-inducible serine/threonine kinase 2; SIK2; SN1L2; SNF1LK2

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK249-3

Antibody Target Type: Pan-specific

Protein UniProt: Q9H0K1

Protein SigNET: Q9H0K1

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human SIK2 (QIK) sequence peptide Cat. No.: PE-01AYR95

Antibody Immunogen Sequence: CLDAVDPQHNGYVLVN

Antibody Immunogen Description: Corresponds to amino acid residues L912 to N926; C-terminus

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Very weak reactivity with recombinant human SIK2.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100-150 kDa.

Antibody Specificity: High

Scientific Background: SIK2 (QIK) is a protein-serine/threonine kinase of the CAMK group and CAMKL family. It is a member of the salt-inducible kinase family. SIK2 may modulate the efficiency of insulin signal transduction, and affect insulin resistancy. It is activated by phosphorylation on T175 by LKB1 (STK11) in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. It stimulates histone deacetylases export. SIK2 is a centrosome kinase required for bipolar mitotic spindle formation, and may be a potential target for therapy in ovarian cancer.
Figure 1. Dot blotting SIK2-3 antibody with recombinant purified proteins.