Target Full Name: Salt-inducible serine/threonine-protein kinase SIK2; Serine-threonine-protein kinase SNF1-like kinase 2

Target Alias: DKFZp434K1115; KIAA0781; LOH11CR1I; Salt-inducible kinase 2; Salt-inducible serine/threonine kinase 2; SIK2; SN1L2; SNF1LK2

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK813

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S358

Protein UniProt: Q9H0K1

Protein SigNET: Q9H0K1

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human SIK2 (QIK) sequence peptide Cat. No.: PE-04APE95

Antibody Immunogen Sequence: RRP(pS)TIA(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues R355 to A361; After the kinase catalytic domain

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.