Product Name: MARK3-BCT
Product Number: AB-NK276-2
Size: 25 µg      Price:89.00
      $US
Target Full Name: MAP/microtubule affinity-regulating protein-serine kinase 3

Target Alias: Cdc25C- associated protein kinase 1; Cdc25C-associated protein kinase 1; CTAK1; C-TAK1; ELKL motif kinase 2; KP78; PAR1A; Protein kinase STK10; Ser/Thr protein kinase PAR-1; Serine/threonine protein kinase p78; Serine/threonine-protein kinase p78

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK276-2

Antibody Target Type: Pan-specific

Protein UniProt: P27448

Protein SigNET: P27448

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human MARK3 sequence peptide Cat. No.: PE-01BDU90

Antibody Immunogen Sequence: RFIKRLPTEYERNGRYEGC

Antibody Immunogen Description: Corresponds to amino acid residues R620 to G637; Pre-C-terminus

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.5 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 80-85 kDa.

Related Product 1: MARK3-BCT blocking peptide

Related Product 2: MARK1-ANT pan-specific antibody (Cat. No.: AB-NK098-2)
Scientific Background: MARK3 is a protein-serine/threonine kinase of the CAMK group and CAMKL family. It has been linked to the specific phosphorylation of microtubule-associated proteins. Phosphorylation of T234 and S238 increases phosphotransferase activity. T211A substitution is involved in decreased phosphorylation and activation by LKB1/LKB1 complex. Phosphorylation of T95 and S96 inhibits phosphotransferase activity.