Product Name: MARK3-pT507
Product Number: AB-PK697
Size: 25 µg      Price:89.00
      $US
Target Full Name: MAP/microtubule affinity-regulating protein-serine kinase 3

Target Alias: Cdc25C- associated protein kinase 1; Cdc25C-associated protein kinase 1; CTAK1; C-TAK1; ELKL motif kinase 2; KP78; PAR1A; Protein kinase STK10; Ser/Thr protein kinase PAR-1; Serine/threonine protein kinase p78; Serine/threonine-protein kinase p78

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK697

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T507

Protein UniProt: P27448

Protein SigNET: P27448

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human MARK3 sequence peptide Cat. No.: PE-04ANR99

Antibody Immunogen Sequence: RRN(pT)YVC(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues R504 to C510; In the C-terminal quarter of the protein kinase. This is the major in vivo phosphorylation site in MARK3.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 80-85 kDa.
Scientific Background: MARK3 is a protein-serine/threonine kinase of the CAMK group and CAMKL family. It has been linked to the specific phosphorylation of microtubule-associated proteins. Phosphorylation of T234 and S238 increases phosphotransferase activity. T211A substitution is involved in decreased phosphorylation and activation by LKB1/LKB1 complex. Phosphorylation of T95 and S96 inhibits phosphotransferase activity.
Figure 1. Epitope mapping of MARK3-pT507 antibody with similar phosphopeptides on dot blots.