Product Name: Pim2-CT
Product Number: AB-NK288-2
Size: 25 µg      Price:89.00
      $US
Target Full Name: Serine/threonine-protein kinase Pim-2

Target Alias: Pim-2h; ENSG00000102096

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK288-2

Antibody Target Type: Pan-specific

Protein UniProt: Q9P1W9

Protein SigNET: Q9P1W9

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human Pim2 sequence peptide Cat. No.: PE-01BES85

Antibody Immunogen Sequence: LDPWMQTPAEDVPLNPSKGC

Antibody Immunogen Description: Corresponds to amino acid residues L282 to G300; C-terminus

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.28 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 34-38 kDa.

Antibody Specificity: Very high

Antibody Cross Reactivity: No significant cross-reactivities detected in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells, except for a very strong 75 kDa protein. No significant cross-reactive proteins detected in diverse mouse tissues.

Related Product 1: Pim2-CT blocking peptide

Related Product 2: Pim2-BKCD pan-specific antibody (Cat. No.: AB-NK288-1)

Related Product 3: Pim2-pT195 phosphosite-specific antibody (Cat. No.: AB-PK761)

Related Product 4: PimSelectide - Pim1 protein kinase substrate peptide

Related Product 5: RPS6 (230-238) KinSub - 40S ribosomal protein S6 (K230-R238, human) peptide; p70S6K protein kinase substrate peptide

Scientific Background: Pim2 is a protein-serine/threonine kinase of the CAMK group and Pim family. The Pim2 protein, along with the related Pim1 and Pim3 proteins, promotes cell survival and proliferation by acting downstream of cytokine and growth factor signalling pathways. It regulates Myc transcription and stabilization by phosphorylation. It also prevents apoptosis by phosphorylating and inactivating the proapoptotic protein Bad. It induces cell survival and proliferation signals via NF-KB cascade and phosphorylation and inhibition of CDKN1A and CDKN1B. Dysregulation of Pim2 can promote cell proliferation and resistance to apoptosis and oncogenesis. The very low frequency of reported somatic mutations in this protein indicates that it may function as a tumour requiring protein (TRP) in many human tumours. Although it is considered as an oncoprotein (OP), it shows a level of bystander mutations typical of most proteins in human cancers. The active form of the protein kinase normally acts to promote tumour cell proliferation. Pim2 is linked to Mantle Cell Lymphomas (LCM), which are non-Hodgkin lymphomas in which is specifically a B-cell lymphoma is also associated the ATM (ataxia telangiectasia mutated) gene.