Product Name: Pim2-pT195

Product Number: AB-PK761

Size:	25 µg		Price:	89.00
			$US

Target Full Name: Serine/threonine-protein kinase Pim-2

Target Alias: Pim-2h; ENSG00000102096

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK761

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T195

Protein UniProt: Q9P1W9

Protein SigNET: Q9P1W9

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human Pim2 sequence peptide Cat. No.: PE-04AOP95

Antibody Immunogen Sequence: EPY(pT)DFD(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues E192 to D198; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 34-38 kDa.

Scientific Background: Pim2 is a protein-serine/threonine kinase of the CAMK group and Pim family. The Pim2 protein, along with the related Pim1 and Pim3 proteins, promotes cell survival and proliferation by acting downstream of cytokine and growth factor signalling pathways. It regulates Myc transcription and stabilization by phosphorylation. It also prevents apoptosis by phosphorylating and inactivating the proapoptotic protein Bad. It induces cell survival and proliferation signals via NF-KB cascade and phosphorylation and inhibition of CDKN1A and CDKN1B. Dysregulation of Pim2 can promote cell proliferation and resistance to apoptosis and oncogenesis. The very low frequency of reported somatic mutations in this protein indicates that it may function as a tumour requiring protein (TRP) in many human tumours. Although it is considered as an oncoprotein (OP), it shows a level of bystander mutations typical of most proteins in human cancers. The active form of the protein kinase normally acts to promote tumour cell proliferation. Pim2 is linked to Mantle Cell Lymphomas (LCM), which are non-Hodgkin lymphomas in which is specifically a B-cell lymphoma is also associated the ATM (ataxia telangiectasia mutated) gene.

Figure 1. Epitope mapping of Pim2-pT195 antibody with similar phosphopeptides on dot blots.

Figure 2. Identification of phosphosites related to Pim2-pT195.