Product Name: ULK3
Product Number: AB-NK355-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Unc-51 (C. elegan) -like kinase 3
Target Alias: Serine/threonine-protein kinase ULK3; EC:2.7.11.1; Ulk3; DKFZP434C131; FLJ90566; ULK3_HUMAN; unc 51 like kinase 3 (C. elegans); ULK3;
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK355-1
Antibody Target Type: Pan-specific
Protein UniProt: Q6PHR2
Protein SigNET: Q6PHR2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide from the N-terminal of Human ULK3 (AA 166-177)
Target Alias: Serine/threonine-protein kinase ULK3; EC:2.7.11.1; Ulk3; DKFZP434C131; FLJ90566; ULK3_HUMAN; unc 51 like kinase 3 (C. elegans); ULK3;
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK355-1
Antibody Target Type: Pan-specific
Protein UniProt: Q6PHR2
Protein SigNET: Q6PHR2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide from the N-terminal of Human ULK3 (AA 166-177)
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a medium ~53 kDa protein band in A549 cell lysates and more weakly in rat and mouse brain lysates.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: A 1:1000 dilution of SPC-642 was sufficient for detection of ULK3 in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: High
Antibody Cross Reactivity: No other prominent cross-reactive proteins in mouse and rat brain lysates or A431 cell lysates except for a ~30 kDa cross-reactive protein.
Related Product 1: ULK1 pan-specific antibody (Cat. No.: AB-NK298-2)
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: A 1:1000 dilution of SPC-642 was sufficient for detection of ULK3 in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: High
Antibody Cross Reactivity: No other prominent cross-reactive proteins in mouse and rat brain lysates or A431 cell lysates except for a ~30 kDa cross-reactive protein.
Related Product 1: ULK1 pan-specific antibody (Cat. No.: AB-NK298-2)
Scientific Background: UNC-51 like kinase 3, ULK3, is a serine-threonine protein kinase that acts as a regulator of SHH signaling and autophagy. Is phosphorylates in vitro GLI2.
References
[1] Malovergan A. et al. (2010) J Biol Chem. 285(39): 30079-30090.
[2] Young A.R. et al. (2009) Genes Dev. 23(7):798-803.
[1] Malovergan A. et al. (2010) J Biol Chem. 285(39): 30079-30090.
[2] Young A.R. et al. (2009) Genes Dev. 23(7):798-803.
© Kinexus Bioinformatics Corporation 2017