Product Name: HSP90
Product Number: AB-NN061-17
| Size: | 50 µg | Price: | 89.00 | |
| $US |
Target Full Name: Heat shock protein HSP 90-beta
Target Alias: HSP84; HSP86; HSP90; HSP89; HSP90Beta; HSP90A; HSP90AA1; HSP90AB1; HSP90B; HSP90N; HSPC1; HSPC2; HSPCA; HSPCAL1; HSPCAL4; HSPCB; HSPN; LAP2
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN061-17
Antibody Target Type: Pan-specific
Protein UniProt: P08238
Protein SigNET: P08238
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2b
Target Alias: HSP84; HSP86; HSP90; HSP89; HSP90Beta; HSP90A; HSP90AA1; HSP90AB1; HSP90B; HSP90N; HSPC1; HSPC2; HSPCA; HSPCAL1; HSPCAL4; HSPCB; HSPN; LAP2
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN061-17
Antibody Target Type: Pan-specific
Protein UniProt: P08238
Protein SigNET: P08238
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2b
Antibody Immunogen Source: Heat shock protein 90 from the water mold Achyla ambisexualis
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a 90 kDa protein in cell and tissue lysates by Western blotting. This antibody is reactive with both the constitutive and the inducible form of HSP90. It does not bind to the native form and does not recognize HSP90 from E.coli or yeast.
Antibody Species Reactivity: Human | Mouse | Rat | Rabbit | Chicken | Achyla | Wheat Germ | Spodoptera frugiperda
Antibody Positive Control: 1 µg/ml of SMC-112 was sufficient for detection of HSP90 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody
Related Product 1: HSP90 complex pan-specific antibody (Cat. No.: AB-NN061-18)
Antibody Potency: Detects a 90 kDa protein in cell and tissue lysates by Western blotting. This antibody is reactive with both the constitutive and the inducible form of HSP90. It does not bind to the native form and does not recognize HSP90 from E.coli or yeast.
Antibody Species Reactivity: Human | Mouse | Rat | Rabbit | Chicken | Achyla | Wheat Germ | Spodoptera frugiperda
Antibody Positive Control: 1 µg/ml of SMC-112 was sufficient for detection of HSP90 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody
Related Product 1: HSP90 complex pan-specific antibody (Cat. No.: AB-NN061-18)
Related Product 2: HSP90 alpha pan-specific antibody (Cat. No.: AB-NN061-19)
Related Product 3: HSP90 alpha/beta pan-specific antibody (Cat. No.: AB-NN061-2)
Related Product 4: HSP90 pan-specific antibody (Cat. No.: AB-NN061-3)
Related Product 5: HSP90 pan-specific antibody (Cat. No.: AB-NN061-4)
Related Product 6: HSP90 (total) pan-specific antibody (Cat. No.: AB-NN061-9)
Related Product 7: HSP90 alpha pan-specific antibody (Cat. No.: AB-NN164)
Related Product 8: HSP90 beta pan-specific antibody (Cat. No.: AB-NN165)
Related Product 9: Hsp90 beta pan-specific antibody (Cat. No.: AB-NN165-1)
Related Product 3: HSP90 alpha/beta pan-specific antibody (Cat. No.: AB-NN061-2)
Related Product 4: HSP90 pan-specific antibody (Cat. No.: AB-NN061-3)
Related Product 5: HSP90 pan-specific antibody (Cat. No.: AB-NN061-4)
Related Product 6: HSP90 (total) pan-specific antibody (Cat. No.: AB-NN061-9)
Related Product 7: HSP90 alpha pan-specific antibody (Cat. No.: AB-NN164)
Related Product 8: HSP90 beta pan-specific antibody (Cat. No.: AB-NN165)
Related Product 9: Hsp90 beta pan-specific antibody (Cat. No.: AB-NN165-1)
Scientific Background: HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (1-4). Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to
date are involved in cell signaling (5-6). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5 When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation.
In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immuno-adsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (7). Looking for more information on HSP90? Visit our new HSP90 Scientific Resource Guide at http://www.HSP90.ca.
References
[1] Arlander SJH et al. (2003) J Biol Chem 278: 52572-52577.
[2] Pearl H et al. (2001) Adv Protein Chem 59:157-186.
[3] Neckers L et al. (2002) Trends Mol Med 8:S55-S61.
[4] Pratt W, Toft D. (2003) Exp Biol Med 228:111-133.
[5] Pratt W, Toft D. (1997) Endocr Rev 18: 306–360.
[6] Pratt WB. (1998) Proc Soc Exptl Biol Med 217: 420–434.
[7] Whitesell L et al. (1994) Proc Natl Acad Sci USA 91: 8324–8328.
[1] Arlander SJH et al. (2003) J Biol Chem 278: 52572-52577.
[2] Pearl H et al. (2001) Adv Protein Chem 59:157-186.
[3] Neckers L et al. (2002) Trends Mol Med 8:S55-S61.
[4] Pratt W, Toft D. (2003) Exp Biol Med 228:111-133.
[5] Pratt W, Toft D. (1997) Endocr Rev 18: 306–360.
[6] Pratt WB. (1998) Proc Soc Exptl Biol Med 217: 420–434.
[7] Whitesell L et al. (1994) Proc Natl Acad Sci USA 91: 8324–8328.
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