Target Full Name: Heat shock protein HSP 90-beta

Target Alias: D6S182; FLJ26984; Heat shock 84 kDa; heat shock 90kD protein 1, beta; heat shock 90kDa protein 1, beta; heat shock protein 90kDa alpha (cytosolic), class B member 1; heat shock protein beta; Heat shock protein HSP 90-beta; HS90B; HSP 84; HSP 90; HSP84; HSP90-BETA; HSP90AB1; HSP90B; HSPC2; HSPCB

Product Type Specific: Heat shock protein phosphosite-specific antibody

Antibody Code: PN520

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y484

Protein UniProt: P08238

Protein SigNET: P08238

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human HSP90AB1 (HSP90B; HSP90-beta) sequence peptide Cat. No.: PE-04AFE99

Antibody Immunogen Sequence: KSI(pY)YIT(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues K481 to T487; In the middle of the HSP90 domain. This is the major in vivo phosphorylation site in HSP90AB1.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.