Product Name: Copper Transporting ATPase 1
Product Number: AB-NN215-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Copper-transporting ATPase 1
Target Alias: ATP7A; ATP 7A; ATPase Cu transporting; DSMAX; FLJ17790; MC1; MC 1; MK; MNK; OHS; Copper pump 1; Menke; OTTHUMP00000062077; SMAX3; ATPase copper transporting alpha polypeptide; ATPase Cu++ transporting alpha polypeptide (Menkes syndrome); Cu++ transporting P type ATPase; Menkes disease associated protein; Menkes syndrome
Product Type Specific: Copper transport protein pan-specific antibody
Antibody Code: NN215-1
Antibody Target Type: Pan-specific
Protein UniProt: Q04656
Protein SigNET: Q04656
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Target Alias: ATP7A; ATP 7A; ATPase Cu transporting; DSMAX; FLJ17790; MC1; MC 1; MK; MNK; OHS; Copper pump 1; Menke; OTTHUMP00000062077; SMAX3; ATPase copper transporting alpha polypeptide; ATPase Cu++ transporting alpha polypeptide (Menkes syndrome); Cu++ transporting P type ATPase; Menkes disease associated protein; Menkes syndrome
Product Type Specific: Copper transport protein pan-specific antibody
Antibody Code: NN215-1
Antibody Target Type: Pan-specific
Protein UniProt: Q04656
Protein SigNET: Q04656
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2b
Antibody Immunogen Source: Synthetic peptide amino acids 42-61 (cytoplasmic C-terminus) of human Copper- transporting ATPase1
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Antibody Immunogen Source: Synthetic peptide amino acids 42-61 (cytoplasmic C-terminus) of human Copper- transporting ATPase1
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: This antibody detects a ~180 kDa protein in rat brain membrane preparations.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-398 was sufficient for detection of Copper-transporting ATPase1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: One cross-reactive protein at ~39 kDa in mouse brain lysates.
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: This antibody detects a ~180 kDa protein in rat brain membrane preparations.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-398 was sufficient for detection of Copper-transporting ATPase1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: One cross-reactive protein at ~39 kDa in mouse brain lysates.
Scientific Background: The copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from the cell. ATP7A (also known as Copper-transporting ATPase 1) functions as a transmembrane copper-trans locating P-type ATPase and plays a vital role in systemic copper absorption in the gut and copper reabsorption in the kidney. Polarized epithelial cells such as Madin-Darby canine kidney cells are a physiologically relevant model for systemic copper absorption and reabsorption in vivo. Although ATP7A is not detectable in most normal tissues, it is expressed in a considerable fraction of many common tumor types. Increased expression of ATP7A renders cells resistant to cisplatin and carboplatin. Mutations in the ATP7A gene result in Menkes disease, which is fatal in early childhood. Mutations in the ATP7B gene lead to the autosomal recessive disorder, Wilson disease, characterized by neurological symptoms and hepatic damage.
References
[1] Samimi G. et al. (2003) Clin. Cancer Res. 9: 5853-9.
[2] Samimi G. et al. (2004) Mol Pharmacol. 66: 25-32.
[3] Greenough M. et al. (2004) Am. J. Physiol. Cell Physiol. 287: C1463-71.
[4] Song, I.S. et al. (2004) Mol. Cancer Ther. 3: 1543-1549.
[5] van Dongen, E.M. et al. (2004) Biochem. Biophys. Res. Commun. 323: 789-795.
[6] Samimi, G. et al. (2004) Mol Pharmacol 66: 25-32.
[7] Morgan, C.T. et al. (2004) J. Biol. Chem. 279: 36363-36371.
[8] Barnes, N. et al. (2005) J. Biol. Chem. [Epub].
[1] Samimi G. et al. (2003) Clin. Cancer Res. 9: 5853-9.
[2] Samimi G. et al. (2004) Mol Pharmacol. 66: 25-32.
[3] Greenough M. et al. (2004) Am. J. Physiol. Cell Physiol. 287: C1463-71.
[4] Song, I.S. et al. (2004) Mol. Cancer Ther. 3: 1543-1549.
[5] van Dongen, E.M. et al. (2004) Biochem. Biophys. Res. Commun. 323: 789-795.
[6] Samimi, G. et al. (2004) Mol Pharmacol 66: 25-32.
[7] Morgan, C.T. et al. (2004) J. Biol. Chem. 279: 36363-36371.
[8] Barnes, N. et al. (2005) J. Biol. Chem. [Epub].
© Kinexus Bioinformatics Corporation 2017