Product Name: VGLUT2
Product Number: AB-NN323-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Vesicular glutamate transporter 2
Target Alias: Differentiation associated BNPI; DNPI; SLC17A6; Solute carrier family 17 member 6; Differentiation associated Na dependent inorganic phosphate cotransporter; Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6; Differentiation associated Na(+) dependent inorganic phosphate cotransporter; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Sodium dependent inorganic phosphate cotransporter
Product Type Specific: Solute carrier protein pan-specific antibody
Antibody Code: NN323-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9P2U8
Protein SigNET: Q9P2U8
Target Alias: Differentiation associated BNPI; DNPI; SLC17A6; Solute carrier family 17 member 6; Differentiation associated Na dependent inorganic phosphate cotransporter; Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6; Differentiation associated Na(+) dependent inorganic phosphate cotransporter; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Sodium dependent inorganic phosphate cotransporter
Product Type Specific: Solute carrier protein pan-specific antibody
Antibody Code: NN323-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9P2U8
Protein SigNET: Q9P2U8
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~60 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-395 was sufficient for detection of VGLut2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Product Use: Western blotting | Immunohistochemistry
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~60 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-395 was sufficient for detection of VGLut2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Related Product 1: VGLUT1 pan-specific antibody (Cat. No.: AB-NN361-1)
Related Product 2: VGLUT3 pan-specific antibody (Cat. No.: AB-NN362-1)
Related Product 2: VGLUT3 pan-specific antibody (Cat. No.: AB-NN362-1)
Scientific Background: The ATP-dependent, chloride-sensitive vesicular glutamate transporters (VGLUT) include BNPI (VGLUT1), VGLUT2 (DNPI) and VGLUT3. The brain expresses BNPI (brain specific Na+-dependent inorganic phosphate (Pi) cotransporter) and VGLUT2 in a complementary fashion. The telencephalic regions express BNPI, whereas the lower brainstem and diencephalic regions express VLGUT2. Rat pinealocytes express both BNPI and VGLUT2. The striatum, hippocampus, cerebral cortex and raphe nuclei express VGLUT3 in a small number of neurons. Pancreatic α and β cells express BNPI and VGLUT2 in response to glucose concentrations. Human VGLUT3 shares a 72% sequence homology with VLGUT2 and BNPI.
References
[1] Aihara Y. et al. (2000) J Neurochem. 74: 2622-2625.
[2] Kaneko T., and Fujiyama F. (2002) Neurosci Res. 42: 243-250.
[3] Gras C. et al. (2002) J Neurosci. 22: 5442-5451.
[4] Takamori S., Malherbe P., Broger C., and Jahn R. (2002) EMBO Rep. 3: 798-803.
[5] Morimoto R., Hayashi M., Yatsushiro S., Otsuka M., Yamamoto A., and Moriyama Y. (2003) J Neurochem. 84: 382-391.
[6] Bai L., Zhang X., and Ghishan F.K. (2003) Am. J. Physiol. Gastrointest. Liver Physiol. 284: G808-814.
[1] Aihara Y. et al. (2000) J Neurochem. 74: 2622-2625.
[2] Kaneko T., and Fujiyama F. (2002) Neurosci Res. 42: 243-250.
[3] Gras C. et al. (2002) J Neurosci. 22: 5442-5451.
[4] Takamori S., Malherbe P., Broger C., and Jahn R. (2002) EMBO Rep. 3: 798-803.
[5] Morimoto R., Hayashi M., Yatsushiro S., Otsuka M., Yamamoto A., and Moriyama Y. (2003) J Neurochem. 84: 382-391.
[6] Bai L., Zhang X., and Ghishan F.K. (2003) Am. J. Physiol. Gastrointest. Liver Physiol. 284: G808-814.
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