Product Name: Sodium-Iodide Symporter
Product Number: AB-NN325-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Sodium/iodide cotransporter
Target Alias: NIS; SLC5A5; solute carrier family 5; Na (+)I(-) cotransporter
Product Type Specific: Sodium-Iodide transport protein pan-specific antibody
Antibody Code: NN325-1
Antibody Target Type: Pan-specific
Protein UniProt: Q92911
Protein SigNET: Q92911
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Mannose binding protein hNIS fusion (AA468-643)
Production Method: Protein G purified
Target Alias: NIS; SLC5A5; solute carrier family 5; Na (+)I(-) cotransporter
Product Type Specific: Sodium-Iodide transport protein pan-specific antibody
Antibody Code: NN325-1
Antibody Target Type: Pan-specific
Protein UniProt: Q92911
Protein SigNET: Q92911
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Mannose binding protein hNIS fusion (AA468-643)
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~97 kDa protein, non-glycosylated version at 68 kDa protein in cell and tissue lysates by Western blotting. Other minor bands associated with hNIS at 160 kDa protein, and degradation products at ~30 kDa protein, and ~15 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-390 was sufficient for detection of hNIS in 20 µg of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Related Product 1: Sodium-Iodide Symporter pan-specific antibody (Cat. No.: AB-NN325-2)
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-390 was sufficient for detection of hNIS in 20 µg of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Related Product 1: Sodium-Iodide Symporter pan-specific antibody (Cat. No.: AB-NN325-2)
Scientific Background: The sodium iodide symporter (NIS) is an ion pump that actively transports iodide across the basolateral membrane into thyroid epithelial cells (1, 2). This is important step in the process of iodide organificaton and the formation of triiodothyronine and thyroxine (3).
References
[1] Dai G., Levy O., Carrasco N. (1996) Nature. 379(6564): 458-460.
[2] Snabik P.A. et al. (1997) Endocrin. 138(8): 3555-3558.
[3] Dohan O. et al. (2007) Proc Natl Acad Sci USA. 104(51): 20250-20255.
[1] Dai G., Levy O., Carrasco N. (1996) Nature. 379(6564): 458-460.
[2] Snabik P.A. et al. (1997) Endocrin. 138(8): 3555-3558.
[3] Dohan O. et al. (2007) Proc Natl Acad Sci USA. 104(51): 20250-20255.
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