Product Name: Sodium-Iodide Symporter
Product Number: AB-NN325-2
Size: 25 µg      Price:89.00
      $US
Target Full Name: Sodium/iodide cotransporter

Target Alias: NIS; SLC5A5; solute carrier family 5; Na (+)I(-) cotransporter

Product Type Specific: Sodium-Iodide Symporter pan-specific antibody

Antibody Code: NN325-2

Antibody Target Type: Pan-specific

Protein UniProt: Q92911

Protein SigNET: Q92911

Antibody Type: Monoclonal

Antibody Host Species: Mouse

Antibody Ig Isotype Clone: IgG1 Kappa

Antibody Immunogen Source: Mannose binding protein hNIS fusion (AA468-643)

Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence

Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.

Antibody Potency: Detects a ~97 kDa protein, non-glycosylated version at 68 kDa protein in cell and tissue lysates by Western blotting. Other minor bands associated with hNIS at 160 kDa protein, and degradation products at ~30 kDa protein, and ~15 kDa protein in cell and tissue lysates by Western blotting.

Antibody Species Reactivity: Human | Mouse | Rat

Antibody Positive Control: 1 µg/ml of SMC-391 was sufficient for detection of hNIS in 20 µg of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Related Product 1: Sodium-Iodide Symporter pan-specific antibody (Cat. No.: AB-NN325-1)

Scientific Background: The sodium iodide symporter (NIS) is an ion pump that actively transports iodide across the basolateral membrane into thyroid epithelial cells (1, 2). This is important step in the process of iodide organificaton and the formation of triiodothyronine and thyroxine (3).