Product Name: AAK1-pS637
Product Number: AB-PK503
Size: 25 µg      Price:89.00
      $US
Target Full Name: AP2-associated protein kinase 1

Target Alias: Adaptor-associated kinase 1; AP2 associated kinase 1; DKFZp686K16132; KIAA1048; Kinase AAK1

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK503

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S637

Protein UniProt: Q2M2I8

Protein SigNET: Q2M2I8

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human AAK1 sequence peptide Cat. No.: PE-04ALP99
Antibody Immunogen Sequence: RIL(pS)DVT(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues R634 to T640; In the C-terminal half of the kinase after the catalytic domain. The major site of phosphorylation of AAK1.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 1 µg/ml for immunoblotting

Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in serum-starved Jurkat cells treated with for 10 minutes with 10% fetal calf serum. Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 90-95 kDa.

Antibody Specificity: High

Antibody Cross Reactivity: Prominent immunreactivities in FBS-treated Jurkat cells at 75 to 120 kDa range.

Related Product 1: AAK1-pS637 blocking peptide

Scientific Background: AAK1 is a protein-serine/threonine kinase of the Other group and NAK family. It stimulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) to ensure high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. AAK1 also targets the phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. It phosphorylates NUMB and promotes NUMB localization to endosomes. It also binds and stabilizes the activated form of NOTCH1 to increases its localization in endosomes. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis.
Figure 1. Epitope mapping of AAK1-pS637 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to AAK1-pS637.