Product Name: CaMK2a-pT286
Product Number: AB-PK555
Size: 25 µg      Price:89.00
      $US
Target Full Name: Calcium/calmodulin-dependent protein-serine kinase 2 alpha

Target Alias: Calcium/calmodulin-dependent protein kinase II alpha; Calcium/calmodulin-dependent protein kinase II alpha-B subunit; Calcium/calmodulin-dependent protein kinase type II alpha chain; CaM kinase II alpha subunit; CaMK2-alpha; CaMKA; CaMK-II alpha subunit; CaMKII-alpha; CaMKIINalpha; Kinase CaMK2-alpha;CaM-kinase II alpha chain; EC 2.7.11.17; KCC2A; KIAA0968

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK555

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T286

Protein UniProt: Q9UQM7

Protein SigNET: Q9UQM7
Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human CaMK2a sequence peptide Cat. No.: PE-04AJB90

Antibody Immunogen Sequence: HRQE(pT)VDC

Antibody Immunogen Description: Corresponds to amino acid residues H282 to C289; In the region between the kinase catalytic domain and the CamKII-AD domains. The major site of in vivo phosphorylation of CaMK2a.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-60 kDa.

Antibody Specificity: High-very high

Antibody Cross Reactivity: Weak cross-reactive proteins of ~36 and ~75 kDa detected in HeLa cells.

Related Product 1: CaMK2a-pT286 blocking peptide

Related Product 2: CaMKII (Alpha-Specific) pan-specific antibody (Cat. No.: AB-NK302-1)

Related Product 3: CaMKII pT286/pT287 phosphosite-specific antibody (Cat. No.: AB-NK302-2)

Related Product 4: Autocamtide-2 peptide - Autocamtide 2 CaMK2-derived peptide substrate

Related Product 5: CAMK2Subtide - CaMK2a protein kinase substrate peptide

Related Product 6: Syntide-2 KinSub - Syntide-2 protein kinase substrate peptide
Scientific Background: CaMK2a is a protein-serine/threonine kinase of the CaMK group and CaMK2 family. It is a calcium/calmodulin-activated protein kinase involved in neurotransmitter release and long term potentiation in neurons. Autophosphorylation of Thr-286 allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state
Figure 1. Dot blotting CaMK2a-pT286 antibody with recombinant purified proteins.


Figure 2. Epitope mapping of CaMK2a-pT286 antibody with similar phosphopeptides on dot blots.


Figure 3. Identification of phosphosites related to CaMK2a-pT286.