Product Name: CDK11A-pT583
Product Number: AB-PK565
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Cell division cycle 2-like 2 protein kinase
Target Alias: CDK19; CD2L6; CDC2L6; CDK8-like; CDK8-like cyclin-dependent kinase; CDK8-like cyclin-dependent kinase 11; Cell division cycle 2-like 6; Cyclin-dependent kinase (CDC2-like) 11; Death-preventing kinase; KIAA1028; CDC2L1; CDK11A; CDK11-p110; CDK11-p46; CDK11-p58; CLK-1; FLJ59152; p58; p58CDC2L1; p58CLK-1; PITSLRE; PK58; Q9UQ88; A4VCI5; Q5QPQ9; Q6P5Y5
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK565
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T583
Protein UniProt: Q9UQ88
Protein SigNET: Q9UQ88
Target Alias: CDK19; CD2L6; CDC2L6; CDK8-like; CDK8-like cyclin-dependent kinase; CDK8-like cyclin-dependent kinase 11; Cell division cycle 2-like 6; Cyclin-dependent kinase (CDC2-like) 11; Death-preventing kinase; KIAA1028; CDC2L1; CDK11A; CDK11-p110; CDK11-p46; CDK11-p58; CLK-1; FLJ59152; p58; p58CDC2L1; p58CLK-1; PITSLRE; PK58; Q9UQ88; A4VCI5; Q5QPQ9; Q6P5Y5
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK565
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T583
Protein UniProt: Q9UQ88
Protein SigNET: Q9UQ88
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDK11A (Cdc2L2; PITSLRE) sequence peptide Cat. No.: PE-04AJJ95
Antibody Immunogen Sequence: KAY(pT)PVV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K580 to V586; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDK11A (Cdc2L2; PITSLRE) sequence peptide Cat. No.: PE-04AJJ95
Antibody Immunogen Sequence: KAY(pT)PVV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K580 to V586; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in sea star oocytes and weaker in Jurkat cells. Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in sea star oocytes and weaker in Jurkat cells. Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 90-100 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactivities detected in Jurkat, Hela, Jurkat and MCF7 cells, but insulin increases 75 kDa in MCF7 cells
Related Product 1: CDK11A-pT583 blocking peptide
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactivities detected in Jurkat, Hela, Jurkat and MCF7 cells, but insulin increases 75 kDa in MCF7 cells
Related Product 1: CDK11A-pT583 blocking peptide
Scientific Background: CDK11 (CDK19, CD2L6; PITSLRE) is a protein-serine/threonine kinase of the CMGC group and CDK family. It is a protein kinase that is a component of Mediator coactivator complex, which is required for transcriptional activation. It has cyclin-dependent protein serine/threonine kinase activity. RT-PCR ELISA detected highest expression was in corpus callosum, followed by kidney and ovary.
Figure 1. Epitope mapping of CDK11A-pT583 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to CDK11A-pT583.
© Kinexus Bioinformatics Corporation 2017