Target Full Name: Cell division protein kinase 12; Cell division cycle 2-related protein kinase 7

Target Alias: CDK12; CD2L7; CDC2 related protein kinase 7; CDC2- related protein kinase 7; Cdc2-related kinase, arginine/serine-rich; CDC2-related protein kinase 7; CRKR; CRKRS; CrkRS; KIAA0904

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK566

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S383+S385

Protein UniProt: Q9NYV4

Protein SigNET: Q9NYV4

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human CDK12 (Cdc2L7) sequence peptide Cat. No.: PE-04ALR99

Antibody Immunogen Sequence: RHS(pS)I(pS)PVR(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues R380 to R388; In the first quarter of the protein. These are This is the major in vivo phosphorylation sites in CDK12.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.