Product Name: Csk-pY184
Product Number: AB-PK589
Size: | 25 µg | | Price: | 89.00 |
| | | $US | |
Target Full Name: C-terminus of Src tyrosine kinase
Target Alias: CYL; MGC117393; MPK-2; CCDS10269.1; ENSG00000103653
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK589
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y184
Protein UniProt: P41240 Protein SigNET: P41240 Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Csk sequence peptide Cat. No.: PE-04AGJ95
Antibody Immunogen Sequence: DEF(pY)RSG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D181 to G187; In the region between the SH2 domain and the protein kinase catalytic domain
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity of a target-sized protein by Western blotting in A431 cells. Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-52 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: Strong ~76 kDa cross-reactive protein detected in A431, HeLa and Jurkat cells, as well as a strong ~180 kDa cross-reactive protein in Jurkat cell.
Related Product 1: Csk-pY184 blocking peptide Related Product 2: CskSubtide - CSK protein kinase substrate peptide Scientific Background: CSK is a protein-tyrosine kinase of the TK group and Csk family. It is a protein kinase that can regulate cell differentiation, growth, and migration, along with mediating immune responses. A Y184F mutation leads to inhibited phosphorylation of CSK. Activity can be decreased by 2/3 with a Y304F mutation. PRKACA induced phosphorylation of CSK can be inhibited with a CSK mutation S364A.
Figure 1. Epitope mapping of Csk-pY184 antibody with similar phosphopeptides on dot blots.
Figure 2. Epitope mapping of Csk-pY184 antibody with similar phosphopeptides on dot blots.
Figure 3. Identification of phosphosites related to Csk-pY184.