Product Name: EphA1-pY781
Product Number: AB-PK605
Size: 25 µg      Price:89.00
      $US
Target Full Name: Ephrin type-A receptor 1 protein-tyrosine kinase

Target Alias: EPA1; Eph; Eph receptor A1; Ephrin type-A receptor 1; EphT; EphT1; MGC163163; Tyrosine-protein kinase receptor Eph; CCDS5884.1; P21709; A1L3V3; ENSG00000146904

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK605

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y781

Protein UniProt: P21709

Protein SigNET: P21709

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human EphA1 sequence peptide Cat. No.: PE-04ALV80

Antibody Immunogen Sequence: DGT(pY)ETQ(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues D778 to Q784; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Medium-strong immunoreactivity of a target-sized protein by Western blotting in HeLa cells.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 95-105 kDa.
Antibody Specificity: Medium

Antibody Cross Reactivity: At least 7 other cross-reactive proteins detected in HeLa cells.

Related Product 1: EphA1-pY781 blocking peptide

Related Product 2: EphA2-pY588 phosphosite-specific antibody (Cat. No.: AB-PK606)

Related Product 3: EphA3-pY779 phosphosite-specific antibody (Cat. No.: AB-PK608)

Scientific Background: EphA1 is a protein-tyrosine kinase of the TK group and Eph family. It is activated by binding of ephrin-A1 on a presenting cell, which is likely to induce dimerization and autophosphorylation. This kinase is moderate to highly expressed in most tested human tissues. Orthologues of EphA1 are highly conserved in mammals and birds. EphA1 has been implicated in mediating developmental events, particularly in the nervous system. EphA1 seems to be a marker of the differentiated normal epidermis, and its downregulation in nonmelanoma skin cancer may contribute to carcinogenesis of these very frequent human tumors. EphA1 represents a new potential prognostic marker and therapeutic target in nonmelanoma skin cancer. EphA1 has also been linked with the development of glioblastomas, head and neck cancer and breast carcinomas (pleomorphic lobular).
Figure 1. Epitope mapping of EphA1-pY781 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to EphA1-pY781.