Product Name: EphB4-pY596
Product Number: AB-PK612
Size: 25 µg      Price:89.00
      $US
Target Full Name: Ephrin type-B receptor 4 protein-tyrosine kinase

Target Alias: EPB4; EPH receptor B4; HTK; Mdk2; MYK1; Tyro11; Tyrosine-protein kinase receptor HTK

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK612

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y596

Protein UniProt: P54760

Protein SigNET: P54760

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human EphB4 sequence peptide Cat. No.: PE-04AMB95
Antibody Immunogen Sequence: PFT(pY)EDP(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues P593 to P599; In the EphA2_TM region between the transmembrane and kinase catalytic domains. This is the major in vivo phosphorylation sites for EphB3.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.08 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100-110 kDa.

Antibody Specificity: High-very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in HeLa and MCF7 cells, except for a very strong ~40 KDa cross-reactive protein in both cells.

Related Product 1: EphB4-pY600 blocking peptide

Scientific Background: EphB4 is a receptor protein-tyrosine kinase of the TK group and Eph family. It is a receptor kinase that will bind the ephrin-B family of ligands (ephrin-A1, B2, or B3) on adjacent cells to mediate cell-contact signalling. EFNB2 binding to EphB4 can be inhibited with a L95R mutation.
Figure 1. Epitope mapping of EphB4-pY596 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to EphB4-pY596.