Product Name: Frk-pY387
Product Number: AB-PK641
Size: | 25 µg | | Price: | 89.00 |
| | | $US | |
Target Full Name: Fyn-related kinase; Tyrosine-protein kinase Frk
Target Alias: Nuclear tyrosine protein kinase RAK; PTK5; RAK; GTK; CCDS5103.1; ENSG00000111816
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK641
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y387
Protein UniProt: P42685 Protein SigNET: P42685 Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Frk sequence peptide Cat. No.: PE-04ARK80
Antibody Immunogen Sequence: EDI(pY)ERH(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E384 to H390; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very weak immunoreactivity of a target-sized protein by Western blotting in HeLa cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Scientific Background: Frk is a protein-tyrosine kinase of the TK group and Src family that is found in the nucleus.. It is involved in positive regulation of PTEN by increasing its stability and preventing degradation and negative regulation of cell proliferation. It may also have functions during G1 and S phase in the nucleus to suppress growth. Frk may be a tumour suppressor protein (TSP). Cancer-related mutations in human tumours point to a loss of function of the protein kinase. nsertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis.
Figure 1. Epitope mapping of Frk-pY387 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to Frk-pY387.