Product Name: Frk-pY497
Product Number: AB-PK642
Size: 25 µg      Price:89.00
      $US
Target Full Name: Fyn-related kinase; Tyrosine-protein kinase Frk

Target Alias: Nuclear tyrosine protein kinase RAK; PTK5; RAK; GTK; CCDS5103.1; ENSG00000111816

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK642

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y497

Protein UniProt: P42685

Protein SigNET: P42685

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human Frk sequence peptide Cat. No.: PE-04AMN95
Antibody Immunogen Sequence: DSS(pY)SDA(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues D494 to A500; In the C-terminus of the kinase. One of the two major in vivo phosphorylation sites in Frk.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.5 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-58 kDa.

Antibody Specificity: High

Antibody Cross Reactivity: No significant cross-reactive proteins detected in Jurkat and T98G cells, except phenylarsine oxide (PAO) treatment increases immunoreactivity of 15 kDa protein in Jurkat cells.

Related Product 1: Frk-pY497 blocking peptide

Related Product 2: Frk-ANT pan-specific antibody (Cat. No.: AB-NK269-1)

Related Product 3: Frk-NT pan-specific antibody (Cat. No.: AB-NK269-2)

Related Product 4: Frk-pY387 phosphosite-specific antibody (Cat. No.: AB-PK641)

Scientific Background: Frk is a protein-tyrosine kinase of the TK group and Src family that is found in the nucleus.. It is involved in positive regulation of PTEN by increasing its stability and preventing degradation and negative regulation of cell proliferation. It may also have functions during G1 and S phase in the nucleus to suppress growth. Frk may be a tumour suppressor protein (TSP). Cancer-related mutations in human tumours point to a loss of function of the protein kinase. nsertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis.
Figure 1. Epitope mapping of Frk-pY497 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to Frk-pY497.