Product Name: Fused-pS159
Product Number: AB-PK643
Size: 25 µg      Price:89.00
      $US
Target Full Name: Serine/threonine-protein kinase 36

Target Alias: FU; Fused serine/threonine kinase; KIAA1278; Serine/threonine kinase 36, fused; STK36; KIAA1278

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK643

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S159

Protein UniProt: Q9NRP7

Protein SigNET: Q9NRP7

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human Fused (STK36) sequence peptide Cat. No.: PE-04AMO99

Antibody Immunogen Sequence: VLT(pS)IKG(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues V156 to G162; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in MCF7 cells that are serum-starved. Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 145-155 kDa.

Antibody Specificity: High-very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in MCF7 and T98G cells or in sea star oocytes, but immunoreactive target band in MCF7 cells extends from 140 to 80 kDa.

Related Product 1: Fused-pS159 blocking peptide

Scientific Background: Fused (STK36) is a protein-serine/threonine kinase of the Other group and ULK family. It functions in the sonic hedgehog (Shh) signalling pathway. Fused regulates the activity of the transcription factors GLI1, GLI2, and GLI3 by opposing SUFU activity and stimulating nuclear translocation of the factors. Fused is also necessary for the assembly of the central pair apparatus in motile cilia. In addition, Fused activity is necessary for post-natal development, potential through its role in ciliary function or regulation of cerebral spinal fluid homeostasis.
Figure 1. Epitope mapping of Fused-pS159 antibody with similar phosphopeptides on dot blots.