Target Full Name: Kinase homologous to SPS1/STE20 (MAP kinase kinase kinase protein-serine kinase 5 (MEKKK5)

Target Alias: GCKR; Germinal center kinase-related; Germinal center kinase-related enzyme; KHS; Kinase KHS1; M4K5; MAP4K5; MAPKKKK5; Q9Y4K4; ENSG00000012983

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK671

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S174

Protein UniProt: Q9Y4K4

Protein SigNET: Q9Y4K4

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human KHS1 (MAP4K5; KHS) sequence peptide Cat. No.: PE-04AND99

Antibody Immunogen Sequence: KRK(pS)FIG(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues K171 to G177; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.