Product Name: Lck-pY263+pY264
Product Number: AB-PK680
Size: 25 µg      Price:89.00
      $US
Target Full Name: Lymphocyte-specific protein-tyrosine kinase; Proto-oncogene tyrosine-protein kinase Lck

Target Alias: LSK; LSK-T; p56-Lck; pp58Lck; RP4-675E8.4; LSK (T-cell specific protein-tyrosine kinase; lck tyrosine kinase; membrane associated protein tyrosine kinase; proto-oncogene Lck; put.ptk; YT16; ENSG00000182866

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK680

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y263+Y264

Protein UniProt: P06239

Protein SigNET: P06239

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human Lck sequence peptide Cat. No.: PE-04ARP55

Antibody Immunogen Sequence: GEVWMG(pY)(pY)NGHT(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues G257 to T268; In the kinase catalytic domain between subdomains I and II.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.5 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-58 kDa.

Related Product 1: Lck-pY263+pY264 blocking peptide

Scientific Background: Lck is a non-receptor protein-tyrosine kinase of the TK group and Src family. Phosphorylation of Y394 increases phosphotransferase activity and induces interaction with Lck. Phosphorylation of Y192 and Y505 inhibits Lck phosphotransferase activity. Lck has a critical function in the selection and maturation of developing T-cells in the thymus, as well as in the function of mature T-cells throughout the body. Lck is a key signalling component of the T-cell antigen receptor (TCR)-linked signal transduction pathway and is constitutively bound to the cytoplasmic domains of the CD4 and CD8 membrane receptors. Lck is expressed through all stages of thymocyte development and is necessary for regulating both pre-TCR and mature alpha beta TCR-mediated maturation. Lck may be an oncoprotein (OP) based on its similarity to other Src family protein-tyrosine kinases.
Figure 1. Epitope mapping of Lck-pY263+pY264 antibody with similar phosphopeptides on dot blots.


Figure 2. Epitope mapping of Lck-pY263+pY264 antibody with similar phosphopeptides on dot blots.


Figure 3. Identification of phosphosites related to Lck-pY263+pY264.