Product Name: MOK-pT159+pY161
Product Number: AB-PK720
Size: | 25 µg | | Price: | 89.00 |
| | | $US | |
Target Full Name: MAPK-MAK-MRK overlapping kinase; MOK protein kinase
Target Alias: RAGE; RAGE1
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK720
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T159+Y161
Protein UniProt: Q9UQ07 Protein SigNET: Q9UQ07 Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MOK (RAGE) sequence peptide Cat. No.: PE-04ABB90
Antibody Immunogen Sequence: QPY(pT)E(pY)IS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues Q156 to S163; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 47-52 kDa.
Scientific Background: MOK is a protein-serine/threonine kinase of the CMGC group and RCK family. Phosphorylation appears to increase its enzymatic activity. MOK belongs to the MAP kinase superfamily, and it may phosphorylate exogenous substrates or autophosphorylate. The protein may functions in regulation of cillium length and intraflagellar transport in renal epithelial cells. MOK may be a tumour requiring protein (TRP), since it displays extremely low rates of mutation in human cancers. MOK is a renal tumour antigen that is silent in most normal tissues except for retina and a number of tumour cell types.
Figure 1. Epitope mapping of MOK-pT159+pY161 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to MOK-pT159+pY161.