Product Name: MOK-AKCD
Product Number: AB-NK281-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: MAPK-MAK-MRK overlapping kinase; MOK protein kinase
Target Alias: RAGE; RAGE1
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK281-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9UQ07
Protein SigNET: Q9UQ07
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MOK (RAGE) sequence peptide Cat. No.: PE-01BEB85
Antibody Immunogen Sequence: EQRKTEKRALGSHRKAGC
Target Alias: RAGE; RAGE1
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK281-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9UQ07
Protein SigNET: Q9UQ07
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MOK (RAGE) sequence peptide Cat. No.: PE-01BEB85
Antibody Immunogen Sequence: EQRKTEKRALGSHRKAGC
Antibody Immunogen Description: Corresponds to amino acid residues E287 to G303; Right after kinase catalytic domain
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in mouse brain, skeletal muscle and thymus, and in human HepG2 cells.
Antibody Species Reactivity: Human
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in mouse brain, skeletal muscle and thymus, and in human HepG2 cells.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 47-52 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No cross-reactivities detected in mouse brain, skeletal muscle, testes and thymus, except for a ~38 kDa protein in mouse skeletal muscle. No significant cross-reactivities detected in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells.
Related Product 1: MOK-AKCD blocking peptide
Related Product 2: MOK-pT159+pY161 phosphosite-specific antibody (Cat. No.: AB-PK720)
Related Product 3: MOK-pY167 phosphosite-specific antibody (Cat. No.: AB-PK721)
Antibody Specificity: Very high
Antibody Cross Reactivity: No cross-reactivities detected in mouse brain, skeletal muscle, testes and thymus, except for a ~38 kDa protein in mouse skeletal muscle. No significant cross-reactivities detected in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells.
Related Product 1: MOK-AKCD blocking peptide
Related Product 2: MOK-pT159+pY161 phosphosite-specific antibody (Cat. No.: AB-PK720)
Related Product 3: MOK-pY167 phosphosite-specific antibody (Cat. No.: AB-PK721)
Scientific Background: MOK is a protein-serine/threonine kinase of the CMGC group and RCK family. Phosphorylation appears to increase its enzymatic activity. MOK belongs to the MAP kinase superfamily, and it may phosphorylate exogenous substrates or autophosphorylate. The protein may functions in regulation of cillium length and intraflagellar transport in renal epithelial cells. MOK may be a tumour requiring protein (TRP), since it displays extremely low rates of mutation in human cancers. MOK is a renal tumour antigen that is silent in most normal tissues except for retina and a number of tumour cell types.
© Kinexus Bioinformatics Corporation 2017