Product Name: RIPK2-pY381
Product Number: AB-PK797
Size: 25 µg      Price:89.00
      $US
Target Full Name: Receptor-interacting serine/threonine-protein kinase 2 (RIPK2)

Target Alias: CARD3; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta converting enzyme associated kinase; CARD-containing interleukin-1 beta-converting enzyme-associated kinase; CARDIAK; GIG30; Kinase RIPK2; Receptor-interacting protein 2; Receptor-interacting serine/threonine protein kinase 2; RICK; RIP2; RIP-like-interacting CLARP kinase

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK797

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y381

Protein UniProt: O43353

Protein SigNET: O43353
Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human RIPK2 (RIP2, RICK) sequence peptide Cat. No.: PE-04APJ90

Antibody Immunogen Sequence: QDA(pY)FMK(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues Q378 to K384; In the region between the kinase catalytic and RHIM domains. This is a major in vivo phosphorylation site in RIPK2.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.75 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-70 kDa.

Antibody Specificity: Very high

Related Product 1: RIPK2-pY381 blocking peptide

Related Product 2: RIPK2-pS176 phosphosite-specific antibody (Cat. No.: AB-PK796)

Scientific Background: RIPK2 (CARD3) is a protein-serine/threonine kinase of the TKL group and RIPK family. It plays an essential role in modulation of innate and adaptive immune responses. It Is activated by phosphorylation at S176.
Figure 1. Epitope mapping of RIPK2-pY381 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to RIPK2-pY381.