Product Name: RIPK2-pS176
Product Number: AB-PK796
Size: 25 µg      Price:89.00
      $US
Target Full Name: Receptor-interacting serine/threonine-protein kinase 2 (RIPK2)

Target Alias: CARD3; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta converting enzyme associated kinase; CARD-containing interleukin-1 beta-converting enzyme-associated kinase; CARDIAK; GIG30; Kinase RIPK2; Receptor-interacting protein 2; Receptor-interacting serine/threonine protein kinase 2; RICK; RIP2; RIP-like-interacting CLARP kinase

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK796

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S176

Protein UniProt: O43353

Protein SigNET: O43353
Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human RIPK2 (RIP2, RICK) sequence peptide Cat. No.: PE-04API75

Antibody Immunogen Sequence: MSL(pS)QSR(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues M173 to R179; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-70 kDa.
Antibody Specificity: High-very high

Antibody Cross Reactivity: Almost no significant cross-reactivities detected in HEK-293, HeLa and T98G cells; in HEK-293 cells, phenylarsine oxide (PAO) decreases 28 kDa protein; in HeLa cells, phenylarsine oxide (PAO) increases 80 + 75 kDa proteins; in T98G cells, PDGF increases 27 + 22 kDa proteins.

Related Product 1: RIPK2-pS176 blocking peptide

Related Product 2: RIPK2-pY381 phosphosite-specific antibody (Cat. No.: AB-PK797)

Scientific Background: RIPK2 (CARD3) is a protein-serine/threonine kinase of the TKL group and RIPK family. It plays an essential role in modulation of innate and adaptive immune responses. It Is activated by phosphorylation at S176.
Figure 1. Epitope mapping of RIPK2-pS176 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to RIPK2-pS176.