Product Name: RSK3-pY217+pS218
Product Number: AB-PK808
Size: | 25 µg | | Price: | 89.00 |
| | | $US | |
Target Full Name: Ribosomal S6 protein-serine kinase 3; Ribosomal protein S6 kinase alpha-3
Target Alias: 90 kDa ribosomal protein S6 kinase 2; HU-2; KS6A2; MAPKAPK1C; p90-RSK3; pp90RSK3; Ribosomal protein S6 kinase alpha 2; Ribosomal protein S6 kinase, 90kDa, polypeptide 2; RPS6KA2; S6K-alpha; S6K-alpha 2
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK808
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y217+S218
Protein UniProt: Q15349 Protein SigNET: Q15349 Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human RSK3 (RPS6KA2) sequence peptide Cat. No.: PE-04AHU95
Antibody Immunogen Sequence: KRA(pY)(pS)FCG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K214 to G221; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in Jurkat cells. Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Scientific Background: RSK3 (RPS6KA2) is a protein-serine/threonine kinase of the AGC group and RSK family. It is a protein-serine/threonine kinase member of the RSK (ribosomal S6 kinase) family of growth factor-regulated kinases. RSK proteins have two kinase catalytic domains and are phosphorylated by MAPK proteins in response to growth factors, polypeptide hormones, and neurotransmitters. RSK3 is activated by multiple phosphorylations on threonine and serine residues. Phosphorylation at S360 increases phosphotransferase activity. Phosphorylation at S218 inhibits interaction with AKAP6. RSK3 acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signalling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. It may function as tumour suppressor in epithelial ovarian cancer cells.
Figure 1. Dot blotting RSK3-pY217+pS218 antibody with recombinant purified proteins.
Figure 2. Epitope mapping of RSK3-pY217+pS218 antibody with similar phosphopeptides on dot blots.
Figure 3. Identification of phosphosites related to RSK3-pY217+pS218.