Product Name: SLK-pS189
Product Number: AB-PK816
Size: 25 µg      Price:89.00
      $US
Target Full Name: STE20-like serine/threonine-protein kinase; CTCL tumour antigen se20-9

Target Alias: bA16H23.1; KIAA0204; LOSK; MGC133067; Se20-9; STE20-like kinase; STK2

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK816

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S189

Protein UniProt: Q9H2G2

Protein SigNET: Q9H2G2

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human SLK sequence peptide Cat. No.: PE-04ALC99

Antibody Immunogen Sequence: RRD(pS)FIG(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues R186 to G192; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.75 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in MCF7 cells. Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 150-200 kDa.

Antibody Specificity: High-very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in HEK-293, HeLa and Jurkat cells, except a 50 kDa protein in A431 cells and 250 kDa protein in HEK-293 and MCF7 cells.

Related Product 1: SLK-pS189 blocking peptide

Related Product 2: SMG1-1 pan-specific antibody (Cat. No.: AB-NK233-2)

Related Product 3: SMG1-2 pan-specific antibody (Cat. No.: AB-NK233-1)

Related Product 4: SMG1-3 pan-specific antibody (Cat. No.: AB-NK233-3)

Scientific Background: SLK is a protein-serine/threonine kinase of the STE group and STE20 family. It may function in apoptosis and actin stress fiber dissolution. SLK was shown to be necessary for ErbB2-driven breast cancer cell motility.
Figure 1. Epitope mapping of SLK-pS189 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to SLK-pS189.