Target Full Name: Serine/threonine-protein kinase SMG1; Lambda/iota protein kinase C-interacting protein

Target Alias: ATX; KIAA0421; Lambda/iota protein kinase C-interacting protein; LIP

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK817

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T3550

Protein UniProt: Q96Q15

Protein SigNET: Q96Q15

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human SMG1 sequence peptide Cat. No.: PE-04APQ99

Antibody Immunogen Sequence: GQK(pT)QPD(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues G3547 to D3553; In the C-terminus of SMG1. This is one of the two major in vivo sites for phosphorylation of SMG1.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.