Product Name: ACTB-pY294
Product Number: AB-PN500
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Actin, cytoplasmic 1
Target Alias: ACTB; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; beta actin; beta cytoskeletal actin; Beta-actin; PS1TP5-binding protein 1; PS1TP5BP1
Product Type Specific: Actin phosphosite-specific antibody
Antibody Code: PN500
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y294
Protein UniProt: P60709
Protein SigNET: P60709
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Target Alias: ACTB; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; beta actin; beta cytoskeletal actin; Beta-actin; PS1TP5-binding protein 1; PS1TP5BP1
Product Type Specific: Actin phosphosite-specific antibody
Antibody Code: PN500
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y294
Protein UniProt: P60709
Protein SigNET: P60709
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ACTB (Beta-actin) sequence peptide Cat. No.: PE-04AEV99
Antibody Immunogen Sequence: KDL(pY)ANT(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K291 to T297; In the Actin domain. This is one of the major in vivo phosphorylation sites in actin.
Antibody Immunogen Sequence: KDL(pY)ANT(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K291 to T297; In the Actin domain. This is one of the major in vivo phosphorylation sites in actin.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium to weak immunoreactivity of a target-sized protein by Western blotting in HEK-293 and Jurkat cells. Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium to weak immunoreactivity of a target-sized protein by Western blotting in HEK-293 and Jurkat cells. Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 42-45 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Weak cross-reactivity with ~27 kDa protein in HEK-293 and Jurkat cells.
Related Product 1: ACTB-pY294 blocking peptide
Related Product 2: ACTB-pY53 phosphosite-specific antibody (Cat. No.: AB-PN501)
Antibody Specificity: High
Antibody Cross Reactivity: Weak cross-reactivity with ~27 kDa protein in HEK-293 and Jurkat cells.
Related Product 1: ACTB-pY294 blocking peptide
Related Product 2: ACTB-pY53 phosphosite-specific antibody (Cat. No.: AB-PN501)
Scientific Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Figure 1. Epitope mapping of ACTB-pY294 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to ACTB-pY294.
© Kinexus Bioinformatics Corporation 2017