Product Name: ACTB-pY53
Product Number: AB-PN501
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Actin, cytoplasmic 1
Target Alias: ACTB; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; beta actin; beta cytoskeletal actin; Beta-actin; PS1TP5-binding protein 1; PS1TP5BP1
Product Type Specific: Actin phosphosite-specific antibody
Antibody Code: PN501
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y53
Protein UniProt: P60709
Protein SigNET: P60709
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Target Alias: ACTB; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; beta actin; beta cytoskeletal actin; Beta-actin; PS1TP5-binding protein 1; PS1TP5BP1
Product Type Specific: Actin phosphosite-specific antibody
Antibody Code: PN501
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y53
Protein UniProt: P60709
Protein SigNET: P60709
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ACTB (Beta-actin) sequence peptide Cat. No.: PE-04AIB95
Antibody Immunogen Sequence: KDS(pY)VGD(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K50 to D56; In the Actin domain. This is one of the major in vivo phosphorylation sites in actin.
Antibody Immunogen Sequence: KDS(pY)VGD(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K50 to D56; In the Actin domain. This is one of the major in vivo phosphorylation sites in actin.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 42-45 kDa.
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 42-45 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: Prominent immunoreactivities in FBS-treated Jurkat cells at 75,to 120 kDa range were detected. No other cross-reactive proteins were observed in Jurkat, HEK-293 and seastar oocytes.
Related Product 1: ACTB-pY53 blocking peptide
Related Product 2: ACTB-pY294 phosphosite-specific antibody (Cat. No.: AB-PN500)
Antibody Cross Reactivity: Prominent immunoreactivities in FBS-treated Jurkat cells at 75,to 120 kDa range were detected. No other cross-reactive proteins were observed in Jurkat, HEK-293 and seastar oocytes.
Related Product 1: ACTB-pY53 blocking peptide
Related Product 2: ACTB-pY294 phosphosite-specific antibody (Cat. No.: AB-PN500)
Scientific Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Figure 1. Epitope mapping of ACTB-pY53 antibody with similar phosphopeptides on dot blots.
Figure 2. Epitope mapping of ACTB-pY53 antibody with similar phosphopeptides on dot blots.
Figure 3. Identification of phosphosites related to ACTB-pY53.
© Kinexus Bioinformatics Corporation 2017