Product Name: ANXA2-pY238
Product Number: AB-PN504
Size: 25 µg      Price:89.00
      $US
Target Full Name: Annexin A2

Target Alias: Annexin II; Annexin-2; ANX2; ANX2L4; anxa 2; ANXA2; CAL1H; Calpactin I heavy chain; calpactin I heavy polypeptide; Calpactin-1 heavy chain; chromobindin 8; Chromobindin-8; LIP2; Lipocortin II; LPC2; LPC2D; p36; PAP-IV; Placental anticoagulant protein IV; Protein I

Product Type Specific: Annexin phosphosite-specific antibody

Antibody Code: PN504

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y238

Protein UniProt: P07355

Protein SigNET: P07355

Antibody Type: Polyclonal

Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ANXA2 sequence peptide Cat. No.: PE-04ACI99

Antibody Immunogen Sequence: YSP(pY)DML(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues Y235 to L241; In the third Annexin domain. The second major in vivo phosphoryation site in Annexin A2.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.5 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 35-40 kDa.
Antibody Specificity: Very high

Antibody Cross Reactivity: No cross-reactive proteins detected in HeLa cells, Jurkat cells and in sea star oocytes.

Related Product 1: ANXA2-pY238 blocking peptide

Related Product 2: ANXA1-pY207 phosphosite-specific antibody (Cat. No.: AB-PN503)

Scientific Background: Annexin A2 is a calcium-regulated membrane-binding protein that binds two calcium ions with high affinity, which is greatly enhanced by anionic phospholipids. It may be involved in heat-stress response and inhibits PCSK9-enhanced LDL receptor degradation.
Figure 1. Epitope mapping of ANXA2-pY238 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to ANXA2-pY238.