Target Full Name: Docking protein 3

Target Alias: Dok-like protein; Dok3; DokL; Downstream of tyrosine kinase 3; FLJ22570; FLJ39939

Product Type Specific: Dok3 phosphosite-specific antibody

Antibody Code: PN508

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y398

Protein UniProt: Q7L591

Protein SigNET: Q7L591

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human Dok3 sequence peptide Cat. No.: PE-04AHH99

Antibody Immunogen Sequence: EHL(pY)ENL(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues E395 to L401; In the last quarter of the protein. This is the major in vivo phosphorylation site in Dok3.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.