Product Name: EEF1A1-pY141
Product Number: AB-PN509
Size: 25 µg      Price:89.00
      $US
Target Full Name: Eukaryotic elongation factor 1-alpha 1

Target Alias: CCS-3; CCS3; cervical cancer suppressor 3; CTCL tumour antigen; EEF-1; EEF1A; eEF1A-1; EEF1A1; EF-1-alpha-1; EF-Tu; EF1A; EF1a-like protein; EF1A1; elongation factor 1 alpha subunit; Elongation factor 1-alpha 1; Elongation factor Tu; Eukaryotic elongation factor 1 A-1; eukaryotic translation elongation factor 1 alpha 1; eukaryotic translation elongation factor 1 alpha 1-like 14; FLJ25721; glucocorticoid receptor AF-1 specific elongation factor; GRAF-1EF; HNGC:16303; LENG7; leukocyte receptor cluster (LRC) member 7; Leukocyte receptor cluster member 7; MGC102687; MGC131894; MGC16224; prostate tumour-inducing protein 1; PTI1; translation elongation factor 1 alpha 1-like 14

Product Type Specific: EEF1A1 phosphosite-specific antibody

Antibody Code: PN509

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y141

Protein UniProt: P68104

Protein SigNET: P68104

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human EEF1A1 sequence peptide Cat. No.: PE-04ACQ90

Antibody Immunogen Sequence: LLA(pY)TLG(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues L138 to G144; In the middle of the GTP_EFTU domain. This is the second major in vivo phosphorylation site in EEF1A1.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.75 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 45-50 kDa.
Antibody Specificity: Very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in HEK-293 and Jurkat cells and sea star oocytes, except for a very strong ~75 kDa protein in FBS-treated Jurkat cells.

Related Product 1: EEF1A1-pY141 blocking peptide

Scientific Background: EEF1A1 promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. It forms a comple with PARP1 and TXK that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.
Figure 1. Epitope mapping of EEF1A1-pY141 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to EEF1A1-pY141.