Product Name: GIT1-pY545
Product Number: AB-PN517
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: ARF GTPase-activating protein GIT1
Target Alias: ARF GAP GIT1; ARF GTPase-activating protein GIT1; CAT-1; CAT1; Cool-associated and tyrosine-phosphorylated protein 1; G protein-coupled receptor kinase interacting ArfGAP 1; G protein-coupled receptor kinase interactor 1; G protein-coupled receptor kinase-interactor 1; GIT1; GRK-interacting protein 1
Product Type Specific: GTPase-activating protein phosphosite-specific antibody
Antibody Code: PN517
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y545
Protein UniProt: Q9Y2X7
Protein SigNET: Q9Y2X7
Antibody Type: Polyclonal
Target Alias: ARF GAP GIT1; ARF GTPase-activating protein GIT1; CAT-1; CAT1; Cool-associated and tyrosine-phosphorylated protein 1; G protein-coupled receptor kinase interacting ArfGAP 1; G protein-coupled receptor kinase interactor 1; G protein-coupled receptor kinase-interactor 1; GIT1; GRK-interacting protein 1
Product Type Specific: GTPase-activating protein phosphosite-specific antibody
Antibody Code: PN517
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y545
Protein UniProt: Q9Y2X7
Protein SigNET: Q9Y2X7
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human GIT1 sequence peptide Cat. No.: PE-04AEN99
Antibody Immunogen Sequence: DAI(pY)SVH(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D542 to H548; In the last third of the protein before the GIT1_C domain. This is the major in vivo phosphorylation site in GIT1.
Antibody Immunogen Source: Human GIT1 sequence peptide Cat. No.: PE-04AEN99
Antibody Immunogen Sequence: DAI(pY)SVH(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D542 to H548; In the last third of the protein before the GIT1_C domain. This is the major in vivo phosphorylation site in GIT1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 85-95 kDa.
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 85-95 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431 and Jurkat cells.
Related Product 1: GIT1-pY545 blocking peptide
Related Product 2: GIT1 pan-specific antibody (Cat. No.: AB-NN261-1)
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431 and Jurkat cells.
Related Product 1: GIT1-pY545 blocking peptide
Related Product 2: GIT1 pan-specific antibody (Cat. No.: AB-NN261-1)
Scientific Background: GIT1 is a GTPase-activating protein for the ADP ribosylation factor family. It may serve as a scaffold to bring together molecules to form signalling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. It increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes.
Figure 1. Epitope mapping of GIT1-pY545 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to GIT1-pY545.
© Kinexus Bioinformatics Corporation 2017