Product Name: ITSN2-pY968
Product Number: AB-PN521
Size: 25 µg      Price:89.00
      $US
Target Full Name: Intersectin-2

Target Alias: ITSN2; KIAA1256; PRO2015; SH3 domain protein 1B; SH3 domain-containing protein 1B; SH3D1B; SH3P18; SH3P18-like WASP associated protein; SH3P18-like WASP-associated protein; SWA; SWAP

Product Type Specific: ITSN2 phosphosite-specific antibody

Antibody Code: PN521

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y968

Protein UniProt: Q9NZM3

Protein SigNET: Q9NZM3

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human ITSN2 sequence peptide Cat. No.: PE-04ADA99

Antibody Immunogen Sequence: EAL(pY)AAV(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues E965 to V971; In the insert region that separates the SH3_2 and SH3_1 domains. This is the second major in vivo phosphorylation site in ITSN2.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in A431 cells and HepG2 cells. Strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 140-170 kDa.

Antibody Specificity: High

Antibody Cross Reactivity: No significant cross-reactive proteins detected in HepG2 and Jurkat cells.

Related Product 1: ITSN2-pY968 blocking peptide

Scientific Background: ITSN2 is an adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. It may regulate the formation of clathrin-coated vesicles (CCPs), and seems to be involved in CCPs maturation including invagination or budding. ITSN2 is involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR). It also plays a role in dendrite formation by melanocytes.
Figure 1. Epitope mapping of ITSN2-pY968 antibody with similar phosphopeptides on dot blots.