Product Name: PLCG1-pY783
Product Number: AB-PN530
Size: 25 µg      Price:89.00
      $US
Target Full Name: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2

Target Alias: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; NCKAP3; Phospholipase C, gamma 1; Phospholipase C-gamma-1; PLC1; PLC148; PLC-148; PLCG-1; PLCgamma1; PLC-gamma1; PLC-II

Product Type Specific: Phospholipase phosphosite-specific antibody

Antibody Code: PN530

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y783

Protein UniProt: P19174

Protein SigNET: P19174

Antibody Type: Polyclonal

Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PLCG1 sequence peptide Cat. No.: PE-04AFD99

Antibody Immunogen Sequence: PGF(pY)VEA(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues P780 to A786; In the intervening region between the second SH2 domain and the SH3 domain in PLCG1. This is one of the major sites of in vivo phosphorylation of PLCG1.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 140-155 kDa.

Related Product 1: PLCG1-pY783 blocking peptide

Scientific Background: PLCG1 mediates the production of the second messenger molecules diacylglycerol (DAG) and 1D-myo-inositol 1,4,5-trisphosphate (IP3) from the hydrolysis of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate. It becomes activated in response to ligand-mediated activation of receptor-type receptor-tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. By virtue of its SH2 domains, PLCG1 binds to tyrosine-phosphorylates receptors and become activated by direct tyrosine phosphorylation by these receptors. It participates in actin reorganization and cell migration.
Figure 1. Epitope mapping of PLCG1-pY783 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to PLCG1-pY783.