Product Name: IKKb-BCT
Product Number: AB-NK076-5
Size: 25 µg      Price:89.00
      $US
Target Full Name: Inhibitor of nuclear factor kappa B kinase beta subunit

Target Alias: I-kappa-B kinase 2; I-kappa-B-kinase beta; IkBKB; IKK2; IKKB; IKK-beta; NFKBIKB; IKBKINASE

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK076-5

Antibody Target Type: Pan-specific

Protein UniProt: O14920

Protein SigNET: O14920

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human IKKb (IkBKB) sequence peptide Cat. No.: PE-01BDG99

Antibody Immunogen Sequence: CTLLENAIQDTVREQDQSFT

Antibody Immunogen Description: Corresponds to amino acid residues C716 to T735; Pre-C-terminus

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.5 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 87-97 kDa.

Related Product 1: IKKb-BCT blocking peptide

Related Product 2: IKKa-pT179+pS180 phosphosite-specific antibody (Cat. No.: AB-PK659)

Related Product 3: IKKb-NT pan-specific antibody (Cat. No.: AB-NK076-6)

Related Product 4: IKKe-pS172 phosphosite-specific antibody (Cat. No.: AB-PK660)

Related Product 5: IKKSelectide - IKKa (CHUK) protein kinase substrate peptide

Scientific Background: IKKb (IKBKB) is a protein-serine/threonine kinase of the Other group and IKK family. It functions in NF-kappa-B signalling and is activated by various indicators of cellular stress, including inflammatory cytokines, bacterial or viral products, and DNA damage. The activity of the kinase enables for NF-kappa-B to enter the nucleus and stimulate the transcription of genes involved in the immune response, control of cell growth, and cellular protection against apoptosis. IKKb may be a tumour requiring protein (TRP), since it displays extremely low rates of mutation in human cancers.
Figure 1. Dot blotting IKKb-BCT antibody with recombinant purified proteins.