Product Name: IKKe-pS172
Product Number: AB-PK660
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Inhibitor of nuclear factor kappa-B kinase subunit epsilon
Target Alias: I kappa-B kinase epsilon; IkBKE; IKKE; IKK-E; IKK-i; IKKI; KIAA0151; RP11-534L20.1; KIAA0151; MGC125294; MGC125295; MGC125297; ENSG00000143466
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK660
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S172
Protein UniProt: Q14164
Protein SigNET: Q14164
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Target Alias: I kappa-B kinase epsilon; IkBKE; IKKE; IKK-E; IKK-i; IKKI; KIAA0151; RP11-534L20.1; KIAA0151; MGC125294; MGC125295; MGC125297; ENSG00000143466
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK660
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S172
Protein UniProt: Q14164
Protein SigNET: Q14164
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human IKKe (IkBKE) sequence peptide Cat. No.: PE-04AMW99
Antibody Immunogen Sequence: KFV(pS)VYG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K169 to G175; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Immunogen Sequence: KFV(pS)VYG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K169 to G175; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in MCF7 cells and sea star oocytes. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in MCF7 cells and sea star oocytes. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 80-87 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in MCF7 and T98G cells.
Related Product 1: IKKe-pS172 blocking peptide
Related Product 2: IKKa-pT179+pS180 phosphosite-specific antibody (Cat. No.: AB-PK659)
Related Product 3: IKKb-BCT pan-specific antibody (Cat. No.: AB-NK076-5)
Related Product 4: IKKb-NT pan-specific antibody (Cat. No.: AB-NK076-6)
Related Product 5: IKKSelectide - IKKa (CHUK) protein kinase substrate peptide
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in MCF7 and T98G cells.
Related Product 1: IKKe-pS172 blocking peptide
Related Product 2: IKKa-pT179+pS180 phosphosite-specific antibody (Cat. No.: AB-PK659)
Related Product 3: IKKb-BCT pan-specific antibody (Cat. No.: AB-NK076-5)
Related Product 4: IKKb-NT pan-specific antibody (Cat. No.: AB-NK076-6)
Related Product 5: IKKSelectide - IKKa (CHUK) protein kinase substrate peptide
Scientific Background: IKKe (IKBKE) is a protein-serine/threonine kinase of the Other group and IKK family. It mediates inflammatory responses in response to viral infections (type I IFN, NF-kB, STAT, TNFa, and IL-1 signalling). Phosphorylation at S172 increases phosphotransferase activity. Ubiquitination and decreased kinase phosphotransferase activity stems from a K30A, or K401A, or K30R, or K401R mutation in IKKe. K30R and K401R will additionally reduce interactions with IKKb, IKBKG, and MyD88. The K38A mutation leads to kinase phosphotransferase activity inhibition and inhibited nuclear transport. Sumoylation and targeting to nuclear bodies is lost with a K231R mutation to IKKe.
Figure 1. Dot blotting IKKe-pS172 antibody with recombinant purified proteins.
Figure 2. Epitope mapping of IKKe-pS172 antibody with similar phosphopeptides on dot blots.
Figure 3. Identification of phosphosites related to IKKe-pS172.
© Kinexus Bioinformatics Corporation 2017